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A nanostructured bacterial bioscaffold for the sustained bottom-up delivery of protein drugs
Seras-Franzoso, Joaquin; Peebo, Karl; Luis Corchero, Jose; Tsimbouri, Penelope M; Unzueta, Ugutz; Rinas, Ursula; Dalby, Matthew J; Vazquez, Esther;...
10.2217/nnm.12.188
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A Novel Arquitectonic Role of Arginine-rich Peptides to Form Nano-disks for Gene Therapy
Vazquez, Esther; Ferrer-Miralles, Neus; Roldan, Monica; Diez-Gil, Cesar; Unzueta, Ugutz; Toledo-Rubio, Veronica; Cedano, Juan; Concillo, Oscar;...
10.1016/j.jbiotec.2010.09.601
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Analytical Approaches for Assessing Aggregation of Protein Biopharmaceuticals
Garcia-Fruitos; Elena Vazquez; Esther Gonzalez-Montalban; Nuria Ferrer-Miralles; Neus Villaverde; Antonio
10.2174/138920111798357339
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Application of Quality by Design to the robust preparation of a liposomal GLA formulation by DELOS-susp method
Merlo-Mas J; Tomsen-Melero J; Corchero JL; González-Mira E; Font A; Pedersen JN; García-Aranda N; Cristóbal-Lecina E; Alcaina-Hernando M; Mendoza...
10.1016/j.supflu.2021.105204
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Bacterial inclusion bodies are industrially exploitable amyloids
de Marco, Ario; Ferrer-Miralles, Neus; Garcia-Fruitos, Elena; Mitraki, Anna; Peternel, Spela; Rinas, Ursula; Trujillo-Roldan, Mauricio A;...
10.1093/femsre/fuy038
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Bacterial inclusion bodies as novel functional and biocompatible nanomaterials
Garcia-Fruitos, E; Rodriguez-Carmona, E; Diez-Gil, C; Ferraz, R M; Vazquez, E; Corchero, J L; Cano-Sarabia, M; Ratera, I; Ventosa, N; Veciana, J;...
10.1016/j.nbt.2009.06.067
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Bacterial Inclusion Bodies: Discovering Their Better Half
Rinas U; Garcia-Fruitós E; Corchero JL; Vázquez E; Seras-Franzoso J; Villaverde A
10.1016/j.tibs.2017.01.005
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Bacterial inclusion bodies: making gold from waste
Garcia-Fruitos; Elena Vazquez; Esther Diez-Gil; Cesar Luis Corchero; Jose Seras-Franzoso; Joaquin Ratera; Imma Veciana; Jaume Villaverde; Antonio
10.1016/j.tibtech.2011.09.003
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Bacterial Inclusion Body Purification
Seras-Franzoso, Joaquin; Peternel, Spela; Cano-Garrido, Olivia; Villaverde, Antonio; Garcia-Fruitos, Elena
10.1007/978-1-4939-2205-5_16
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Bacterially produced inclusion bodies as biocompatible materials for substrate-dependent mammalian cell proliferation
Seras, J; Diez-Gil, C; Vazquez, E; Krabbenborg, S; Rodriguez-Carmona, E; Corchero, J L; Ferraz, R M; Cano-Sarabia, M; Ratera, I; Ventosa, N; Cano, O;...
10.1016/j.jbiotec.2010.09.614
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Bioadhesiveness and efficient mechanotransduction stimuli synergistically provided by bacterial inclusion bodies as scaffolds for tissue engineering
Seras-Franzoso; Joaquin Diez-Gil; Cesar Vazquez; Esther Garcia-Fruitos; Elena Cubarsi; Rafael Ratera; Imma Veciana; Jaume Villaverde; Antonio
10.2217/NNM.11.83
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Biological role of bacterial inclusion bodies: a model for amyloid aggregation
Garcia-Fruitos; Elena Sabate; Raimon de Groot; Natalia S Villaverde; Antonio Ventura; Salvador
10.1111/j.1742-4658.2011.08165.x
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BIOMEDICAL APPLICATIONS OF BACTERIAL INCLUSION BODIES
Ratera, Imma; Peternel, Spela; Seras-Franzoso, Joaquin; Cano-Garrido, Olivia; Garcia-Fruitos, Elena; Cubarsi, Rafael; Vazquez, Esther; Luis Corchero,...
10.1002/9781118845363
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Bottom-Up Instructive Quality Control in the Biofabrication of Smart Protein Materials
Rueda; Fabian Virtudes Cespedes; Maria Conchillo-Sole; Oscar Sanchez-Chardi; Alejandro Seras-Franzoso; Joaquin Cubarsi; Rafael Gallardo; Alberto...
10.1002/adma.201503676
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Cellular uptake and intracellular fate of protein releasing bacterial amyloids in mammalian cells
Seras-Franzoso J; Sánchez-Chardi A; Garcia-Fruitós E; Vázquez E; Villaverde A
10.1039/c5sm02930a
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Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control
Platas; Gemma Rodriguez-Carmona; Escarlata Garcia-Fruitos; Elena Cano-Garrido; Olivia Villaverde; Antonio
10.1186/1475-2859-10-79
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Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
Torrealba, D; Seras-Franzoso, J; Mamat, U; Wilke, K; Villaverde, A; Roher, N; Garcia-Fruitos, E
10.1371/journal.pone.0164073
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CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis
Cano-Garrido, O; Cespedes, MV; Unzueta, U; Saccardo, P; Roldan, M; Sanchez-Chardi, A; Cubarsi, R; Vazquez, E; Mangues, R; Garcia-Fruitos, E;...
10.2217/nnm-2016-0200
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Disulfide Bond Formation and Activation of Escherichia coli beta-Galactosidase under Oxidizing Conditions
Seras-Franzoso; Joaquin Affentranger; Roman Ferrer-Navarro; Mario Daura; Xavier Villaverde; Antonio Garcia-Fruitos; Elena
10.1128/AEM.06923-11
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Divergent genetic control of protein solubility and conformational quality in Escherichia coli
Garcia-Fruitos; Elena Martinez-Alonso; Monica Gonzalez-Montalban; Nuria Valli; Minoska Mattanovich; Diethard Villaverde; Antonio
10.1016/j.jmb.2007.09.004
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Editorial: High added-value nanoparticles: Rethinking and recycling cell protein waste
Unzueta, U; Sanchez, JM; Garcia-Fruitos, E; Seras-Franzoso, J
10.3389/fbioe.2022.1018014
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Engineering bacterial inclusion bodies as nanostructured depots of functional protein drugs
Villaverde, Antonio; Virtudes Cespedes, Maria; Fernandez, Yolanda; Unzueta, Ugutz; Mendoza, Rosa; Seras-Franzoso, Joaquin; Cano-Garrido, Olivia;...
10.1016/j.nbt.2016.06.1237
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Engineering protein self-assembling in protein-based nanomedicines for drug delivery and gene therapy
Ferrer-Miralles, Neus; Rodriguez-Carmona, Escarlata; Corchero, Jose Luis; Garcia-Fruitos, Elena; Vazquez, Esther; Villaverde, Antonio
10.3109/07388551.2013.833163
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Engineering tumor cell targeting in nanoscale amyloidal materials
Unzueta, U; Seras-Franzoso, J; Cespedes, MV; Saccardo, P; Cortes, F; Rueda, FB; Garcia-Fruitos, E; Ferrer-Miralles, N; Mangues, R; Vazquez, E;...
10.1088/0957-4484/28/1/015102
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Evaluation of bacterial lysis procedures for the efficient release of cellular proteins and polymers of biomedical interest
Garcia-Fruitos, E; Mendoza, R; Villaverde, A; Rodriguez-Carmona, E
10.1016/j.jbiotec.2010.09.483
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Expanding the recombinant protein quality in Lactococcus lactis
Cano-Garrido; Olivia Rueda; Fabian L Sanchez-Garcia; Laura Ruiz-Avila; Luis Bosser; Ramon Villaverde; Antonio Garcia-Fruitos; Elena
10.1186/s12934-014-0167-3
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Extracellular vesicles increase the enzymatic activity of lysosomal proteins and improve the efficacy of enzyme replacement therapy in Fabry disease
Abasolo, Bane; Seras-Franzoso, Joaquin; Vanessa Diaz-Riascos, Zamira; Luis Corchero, Jose; Gonzalez, Patricia; Garcia-Aranda, Natalia; Mandana,...
10.1016/j.ymgme.2019.11.010
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Friendly production of bacterial inclusion bodies
Garcia-Fruitos; Elena Villaverde; Antonio
10.1007/s11814-010-0161-3
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Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies
Vazquez, Esther; Corchero, Jose L; Burgueno, Joan F; Seras-Franzoso, Joaquin; Kosoy, Ana; Bosser, Ramon; Mendoza, Rosa; Martinez-Lainez, Joan Marc;...
10.1002/adma.201104330
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Functional inclusion bodies produced in the yeast Pichia pastoris
Rueda, F; Gasser, B; Sanchez-Chardi, A; Roldan, M; Villegas, S; Puxbaum, V; Ferrer-Miralles, N; Unzueta, U; Vazquez, E; Garcia-Fruitos, E;...
10.1186/s12934-016-0565-9
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Functional protein aggregates: just the tip of the iceberg
Villaverde, A; Corchero, JL; Seras-Franzoso, J; Garcia-Fruitos, E
10.2217/nnm.15.125
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Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery
Seras-Franzoso, Joaquin; Steurer, Christoph; Roldan, Monica; Vendrell, Meritxell; Vidaurre-Agut, Carla; Tarruella, Anna; Saldana, Laura; Vilaboa,...
10.1016/j.jconrel.2013.06.034
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General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins
Ferrer-Miralles, Neus; Saccardo, Paolo; Luis Corchero, Jose; Xu, Zhikun; Garcia-Fruitos, Elena
10.1007/978-1-4939-2205-5_1
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High-Throughput Cell Motility Studies on Surface-Bound Protein Nanoparticles with Diverse Structural and Compositional Characteristics
Tatkiewicz, Witold I; Seras-Franzoso, Joaquin; Garcia-Fruitos, Elena; Vazquez, Esther; Kyvik, Adriana R; Ventosa, Nora; Guasch, Judith; Villaverde,...
10.1021/acsbiomaterials.9b01085
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Improved performance of protein-based recombinant gene therapy vehicles by tuning downstream procedures
Unzueta, Ugutz; Saccardo, Paolo; Ferrer-Miralles, Neus; Garcia-Fruitos, Elena; Vazquez, Esther; Villaverde, Antonio; Cortes, Francisco; Mangues, Ramon
10.1002/btpr.1798
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Improving Biomaterials Imaging for Nanotechnology: Rapid Methods for Protein Localization at Ultrastructural Level
Cano-Garrido, O; Garcia-Fruitos, E; Villaverde, A; Sanchez-Chardi, A
10.1002/biot.201700388
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Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates
Seras-Franzoso; Joaquin Peebo; Karl Garcia-Fruitos; Elena Vazquez; Esther Rinas; Ursula Villaverde; Antonio
10.1016/j.actbio.2013.12.021
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In progress
Fernando Rodríguez (Codirector) ; Jordi Argilaguet (Codirector) ; GARCIA FRUITOS, ELENA (Codirector) Doctorando: Aida Tort Miró
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In situ protein folding and activation in bacterial inclusion bodies
Gonzalez-Montalban; Nuria Natalello; Antonino Garcia-Fruitos; Elena Villaverde; Antonio Doglia; Silvia Maria
10.1002/bit.21797
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Inclusion bodies of fuculose-1-phosphate aldolase as stable and reusable biocatalysts
Sans; Cristina Garcia-Fruitos; Elena Ferraz; Rosa M Gonzalez-Montalban; Nuria Rinas; Ursula Lopez-Santin; Josep Villaverde; Antonio Alvaro; Gregorio
10.1002/btpr.1518
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Inclusion bodies: a new concept
Garcia-Fruitos, Elena
10.1186/1475-2859-9-80
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Inclusion body-like organization of protein-based artificial viruses for gene therapy
Domingo-Espin, J; Vazquez, E; Ganz, J; Conchillo, O; Garcia-Fruitos, E; Cedano, J; Unzueta, U; Gonzalez-Montalban, N; Daura, X; Peluffo, H;...
10.1016/j.jbiotec.2010.09.621
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Insoluble Proteins Methods and Protocols Preface
Garcia-Fruitos, Elena
10.1007/978-1-4939-2205-5
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Integrating mechanical and biological control of cell proliferation through bioinspired multieffector materials
Seras-Franzoso; Joaquin Tatkiewicz; Witold I Vazquez; Esther Garcia-Fruitos; Elena Ratera; Imma Veciana; Jaume Villaverde; Antonio
10.2217/NNM.15.5
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Isolation of cell-free bacterial inclusion bodies
Rodriguez-Carmona; Escarlata Cano-Garrido; Olivia Seras-Franzoso; Joaquin Villaverde; Antonio Garcia-Fruitos; Elena
10.1186/1475-2859-9-71
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Lactic acid bacteria: a promising alternative for recombinant protein production
Garcia-Fruitos, Elena
10.1186/1475-2859-11-157
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Lactic acid bacteria: reviewing the potential of a promising delivery live vector for biomedical purposes
Cano-Garrido, O; Seras-Franzoso, J; Garcia-Fruitos, E
10.1186/s12934-015-0313-6
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Learning about protein solubility from bacterial inclusion bodies
Martinez-Alonso, Monica; Gonzalez-Montalban, Nuria; Garcia-Fruitos, Elena; Villaverde, Antonio
10.1186/1475-2859-8-4
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Nanoparticulate architecture of protein-based artificial viruses is supported by protein-DNA interactions
Domingo-Espin, Joan; Vazquez, Esther; Ganz, Javier; Conchillo, Oscar; Garcia-Fruitos, Elena; Cedano, Juan; Unzueta, Ugutz; Petegnief, Valerie;...
10.2217/nnm.11.28
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Nanostructured recombinant cytokines: A highly stable alternative to short-lived prophylactics
Torrealba, D; Parra, D; Seras-Franzoso, J; Vallejos-Vidal, E; Yero, D; Gibert, I; Villaverde, A; Garcia-Fruitos, E; Roher, N
10.1016/j.biomaterials.2016.08.043
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Non-amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles
Unzueta; Ugutz Ferrer-Miralles; Neus Cedano; Juan Xu Zikung Pesarrodona; Mireia Saccardo; Paolo Garcia-Fruitos; Elena Domingo-Espin; Joan Kumar;...
10.1016/j.biomaterials.2012.08.033
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Packaging protein drugs as bacterial inclusion bodies for therapeutic applications
Villaverde, Antonio; Garcia-Fruitos, Elena; Rinas, Ursula; Seras-Franzoso, Joaquin; Kosoy, Ana; Luis Corchero, Jose; Vazquez, Esther
10.1186/1475-2859-11-76
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Production of functional inclusion bodies in endotoxin-free Escherichia coli
Rueda; Fabian Cano-Garrido; Olivia Mamat; Uwe Wilke; Kathleen Seras-Franzoso; Joaquin Garcia-Fruitos; Elena Villaverde; Antonio
10.1007/s00253-014-6008-9
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Production of self-organizing protein nanomaterials in endotoxin-free Escherichia coli
Rueda Alfonso, Fabian; Virtudes Cespedes, Maria; Cano Garrido, Olivia; Sanchez Chardi, Alejandro; Ferrer Miralles, Neus; Vazquez, Esther; Unzueta,...
10.1016/j.nbt.2016.06.925
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Protein Aggregation and Soluble Aggregate Formation Screened by a Fast Microdialysis Assay
Toledo-Rubio; Veronica Vazquez; Esther Platas; Gemma Domingo-Espin; Joan Unzueta; Ugutz Steinkamp; Elke Garcia-Fruitos; Elena Ferer-Mirales; Neus...
10.1177/1087057110363911
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Recombinant protein materials for bioengineering and nanomedicine
Luis Corchero; Jose Vazquez; Esther Garcia-Fruitos; Elena Ferrer-Miralles; Neus Villaverde; Antonio
10.2217/NNM.14.153
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Recombinant protein solubility - does more mean better?
Gonzalez-Montalban; Nuria Garcia-Fruitos; Elena Villaverde; Antonio
10.1038/nbt0707-718
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Sheltering DNA in self-organizing, protein-only nano-shells as artificial viruses for gene delivery
Unzueta, Ugutz; Saccardo, Paolo; Domingo-Espin, Joan; Cedano, Juan; Conchillo-Sole, Oscar; Garcia-Fruitos, Elena; Cespedes, Maria Virtudes; Corchero,...
10.1016/j.nano.2013.11.006
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Side effects of chaperone gene co-expression in recombinant protein production
Martinez-Alonso; Monica Garcia-Fruitos; Elena Ferrer-Miralles; Neus Rinas; Ursula Villaverde; Antonio
10.1186/1475-2859-9-64
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Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance
Martinez-Miguel, Marc; Kyvik, Adriana R; Ernst, Lena M; Martinez-Moreno, Albert; Cano-Garrido, Olivia; Garcia-Fruitos, Elena; Vazquez, Esther;...
10.1039/d0tb00702a
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Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
Rueda F; Céspedes MV; Sánchez-Chardi A; Seras-Franzoso J; Pesarrodona M; Ferrer-Miralles N; Vázquez E; Rinas U; Unzueta U; Mamat U; Mangues R;...
10.1186/s12934-016-0457-z
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Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies
Cano-Garrido, Olivia; Rodriguez-Carmona, Escarlata; Diez-Gil, Cesar; Vazquez, Esther; Elizondo, Elisa; Cubarsi, Rafael; Seras-Franzoso, Joaquin;...
10.1016/j.actbio.2012.11.033
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Surface Cell Growth Engineering Assisted by a Novel Bacterial Nanomaterial
Garcia-Fruitos; Elena Rodriguez-Carmona; Escarlata Diez-Gil; Cesar Ferraz; Rosa M Vazquez; Esther Luis Corchero; Jose Cano-Sarabia; Mary Ratera; Imma...
10.1002/adma.200900283
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Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
Tatkiewicz; Witold I Seras-Franzoso; Joaquin Garcia-Fruitos; Elena Vazquez; Esther Kyvik; A R Guasch; Judith Villaverde; Antonio Veciana; Jaume...
10.1021/acsami.8b06821
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Systems-Level Analysis of Protein Quality in Inclusion Body-Forming Escherichia coli Cells
Garcia-Fruitos, Elena; Gonzalez-Montalban, Nuria; Martinez-Alonso, Monica; Rinas, Ursula; Villaverde, Antonio
10.1007/978-1-4020-9394-4_15
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The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum
Martinez-Alonso; Monica Gonzalez-Montalban; Nuria Garcia-Fruitos; Elena Villaverde; Antonio
10.1128/AEM.01446-08
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The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation
Diez-Gil; Cesar Krabbenborg; Sven Garcia-Fruitos; Elena Vazquez; Esther Rodriguez-Carmona; Escarlata Ratera; Imma Ventosa; Nora Seras-Franzoso;...
10.1016/j.biomaterials.2010.04.008
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Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces
Seras-Franzoso, Joaquin; Tsimbouri, Penelope M; Burgess, Karl V; Unzueta, Ugutz; Garcia-Fruitos, Elena; Vazquez, Esther; Villaverde, Antonio; Dalby,...
10.2217/nnm.13.43
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Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering
Garcia-Fruitos; Elena Seras-Franzoso; Joaquin Vazquez; Esther Villaverde; Antonio
10.1088/0957-4484/21/20/205101
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Two-dimensional microscale engineering of protein-based nanoparticles for cell guidance
Tatkiewicz, Witold I; Seras-Franzoso, Joaquin; Garcia-Fruitos, Elena; Vazquez, Esther; Ventosa, Nora; Peebo, Karl; Ratera, Imma; Villaverde, Antonio;...
10.1021/nn400907f
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Variant Morphologies of Bacterial Inclusion Bodies Improve Substrate Material Properties that Support Mammalian Cell Proliferation
Garcia-Fruitos, Elena; Seras, Joaquin; Vazquez, Esther; Villaverde, Antonio
10.1016/j.jbiotec.2010.09.613
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Yield, Solubility and Conformational Quality of Soluble Proteins Are Not Simultaneously Favored in Recombinant Escherichia coli
Martinez-Alonso; Monica Garcia-Fruitos; Elena Villaverde; Antonio
10.1002/bit.21996
